Journal: The Pharmacogenomics Journal

Article Title: A TOMM40 variable-length polymorphism predicts the age of late-onset Alzheimer's disease

doi: 10.1038/tpj.2009.69

Figure Lengend Snippet: Schematic overview of the TOMM40-APOE locus. ( a ) Genomic locations of the TOMM40 and APOE genes on chromosome 19 between 50 084 480 and 50 107 480 bp. The regions subjected to primary sequencing and phylogenetic analysis for the exploratory (R1) (23 kb) and confirmatory (R2) (10 kb) studies are highlighted on the genomic map (NCBI Build 36.3). ( b ) Distribution of SNP and insertion/deletion polymorphisms are shown on the gene structure of TOMM40 covered in the region that was subjected to primary sequencing. The region covers exons 6–10 and all associated intronic regions. The variable poly-T repeat (rs10524523) that is significantly associated with LOAD age of onset is depicted with the square labeled ‘P'.

Article Snippet: A proprietary sequencing analysis program called ‘Agent' (developed by Celera, Alameda, CA, USA) was used to align sequencing reads to the appropriate reference sequence, and produce ‘contigs' associated with each clone.

Techniques: Sequencing, Labeling